Figure 5.

Alignment of HMG-CoA reductase sequences around the critical catalytic residues. Four regions of sequence around the catalytic residues of P. mevalonii HMG-CoA reductase (Glu83, Lys267, Asp283, and His381) are shown in comparison to selected sequences from a variety of biosynthetic HMG-CoA reductases. The numbering for P. mevalonii is shown in bold at the bottom. The numbering at the top of the figure is that for the human enzyme, given as a reference. Catalytic residues are indicated by white on black lettering. Conserved residues are shaded in gray. Site-directed mutagenesis of the P. mevalonii and Syrian hamster enzymes have confirmed that the aligned residues are catalytic homologues.