Figure 3.
Mining for genes expressed in patterns consistent with primitive streak, and epiblast differentiation programs. (A) GeneSpring representation of epiblast and streak gene expression profiles during EB differentiation in 10% serum or serum-freeB4L culture. The up-regulation of genes between days 2–4 were similar under both conditions. (B) qRT-PCR analysis of Mixl, Lim1, Cdx4 and Riken clone 8430415E04Rik. The Y-axis represents expression relative to the housekeeping gene HPRT. Error bars indicate ± SD from three biological replicates. (C) 102 genes transiently up regulated during the first 4 days of ES cell differentiation were clustered using a tree algorithm and Pearson correlation of > 0.9. The 'Brief' group represents gene with very transient expression at day 3. (D) Coding region for 8430415E04Rik. The shaded areas represent the three Heat domains.