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Results from Real-time PCR
| Gene | Real-time PCR fold change | |
| ACTB | 18S | |
| CYP17A1 | 2.28 (p = 0.0015) | 2.87 (p = 0.001) |
| CYB5 | 2.24 (p = 0.08) | 2.84 (p = 0.025) |
| FTL | 1.88 (p = 0.078) | 2.58 (p = 0.0026) |
| EEF1A1 | 0.95 (p = 0.77) | 1.30 (p = 0.23) |
Real-time PCR was used to confirm expression variations of selected genes from the microarray study. Normalisation of the values was performed by using ACTB and 18S as control genes. Three of four examined genes were verified and one was contradicted.
