Table 5.
Gene | Fold | Log10 Fold change | Bias | Std error | P value |
SMPD1 | 1.4 | 0.16 | -1.60 | 0.18 | 0.185 |
StAR | 12.8 | 1.11 | -2.65 | 0.13 | 0.000 |
SULT2A1 | 2.4 | 0.38 | -4.37 | 0.11 | 0.002 |
HSD17B4 | 1.7 | 0.23 | -2.13 | 0.09 | 0.006 |
AKR1C4 | 2.5 | 0.39 | -2.07 | 0.09 | 0.000 |
CYP11A1 | 3.2 | 0.50 | 5.84 | 0.12 | 0.000 |
rcPCR was used to confirm expression variations of selected genes from the microarray study. Fold changes are calculated relative to baseline, which is the group of low androstenone (NLL) in this case, and adjusted for the housekeeping gene HPRT. Five of six examined genes were verified and one was contradicted.