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. Author manuscript; available in PMC: 2008 Jan 17.
Published in final edited form as: Thromb Res. 2006 Oct 18;120(2):259–268. doi: 10.1016/j.thromres.2006.09.005

FIGURE 5. Immunoblot with PLA2 isoforms.

FIGURE 5

Immunoblot analysis of human iPLA2-beta (left) and -gamma (right) in cytosolic and membrane subcellular fractions and whole platelets. Proteins were separated by SDS-PAGE and transferred to nitrocellulose. Membranes were probed with anti-iPLA2-beta or-gamma antibodies (1:1000 dilution) and incubated with horseradish peroxidase-linked secondary antibodies (1:10,000 dilution). Immunoblots were detected with enhanced chemiluminescence and exposure to film for 5 minutes.