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. 2006 Jun 7;26(23):6172–6180. doi: 10.1523/JNEUROSCI.0447-06.2006

Figure 5.

Figure 5.

Targeting of PMCA2w/a and PMCA2w/b transfected in rat hair cells. A, Bundle-specific PMCA2w/a expression in outer hair cells labeled with anti-C8 for epitope-tagged PMCA2w/a (green), GFP-actin (blue), and phalloidin to detect actin (red). Arrows indicate cortical actin from the GFP-actin signal in a transfected cell; note the absence of the C8-PMCA2w/a signal in the corresponding panel. The white color of the hair bundle in the last panel indicates overlap of PMCA2w/a, GFP-actin, and phalloidin signals. B, Hair-bundle targeting of PMCA2w/a in a utricular hair cell. C, Hair-bundle targeting of PMCA2w/b in a cochlear outer hair cell. D, Hair-bundle targeting of PMCA2w/b in a utricular hair cell. Scale bars, 5 μm.