Figure 7.

Expression of GFP-PMCA2w/a in rat hair cells. Representative hair cells transfected with GFP-PMCA2w/a alone (A–H) or with GFP-PMCA2w/a and C8-actin (I–L). A–D, Vestibular hair cell transfected with GFP-PMCA2w/a (green) and labeled with phalloidin to detect actin (red). A, x–z projection. B–D, Individual sections at levels indicated in A. E–H, Cochlear outer hair cell transfected as in A–D. I–L, Cochlear outer hair cell transfected with GFP-PMCA2w/a (green) and C8-actin (blue); actin was detected with phalloidin (red). Scale bars: A, E, I, 5 μm; (in B, F, J) B–D, F–H, J–L, 5 μm.