Table 2.

Hair-bundle enrichment of PMCA2 splice forms

Isoform	Bundle/basolateral membrane fluorescence (mean ± SEM)	Number of cells analyzed	Significance relative to PMCA2w/a
C8-PMCA2w/a	31 ± 6	27
C8-PMCA2z/a	0.26 ± 0.08	8	0.01
C8-PMCA2w/b	9 ± 2	3	0.19
C8-PMCA2w-AAA/a	14 ± 6	4	0.37
GFP-PMCA2w/a	3.0 ± 0.6	16	0.001

The fluorescence intensity of hair bundles and the basolateral membrane of transfected hair cells was compared with the intensity in nearby, untransfected cells. There were no statistically significant differences between auditory and vestibular hair cells. Conditions were compared statistically using the two-tailed t test, assuming equal variance.