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Triketide lactone formation assay. (A) Chain elongation reaction catalyzed by a KS–AT didomain interacting with a methylmalonyl-ACP. The KS domain is preacylated with the electrophilic diketide substrate (NDK). (B) TLC phosphorimaging results, showing the amount of 14C-labeled triketide lactone product in each reaction at the indicated time points.
