Figure 2.

The kinetics of amyloid fibril formation. WT rPrP (10 μM) (▪), Q218K rPrP (10 μM) (▾), or a 1:1 WT:Q218K rPrPs mixture (total protein concentration of 20 μM) (•) was incubated in 50 mM MES at pH 6.0, 2 M GdnHCl at 37°C upon continuous shaking at 600 rpm and analyzed using ThT fluorescence assay. The ThT fluorescence reading was normalized per 0.3 μM/mL rPrP present in the ThT assay mixture.