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Thermostability of tmGBP. (A) Thermal denaturation of tmGBP in 6 M guanidine hydrogen chloride: (•) in the absence of glucose; (▪) in the presence of 1 mM glucose. Continuous lines are fit to a two-state transition model (Schellman 1987). (B) Thermostability in buffer was obtained by linear extrapolation of melting transition midpoint determined: (•) in the absence of glucose; (▪) in the presence of 1 mM glucose. Straight lines represent linear fits to the data.
