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Fluorescent response of the Y13C•Cy5 conjugate upon glucose titration (20 mM MOPS and 150 mM NaCl, pH 7.0, 37°C). (Inset) Emission spectra of this conjugate in the absence (solid line) and presence (dashed line) of 140 mM glucose. (Bottom) Fit to a hyperbolic binding isotherm.
