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. 1999 Jun 22;96(13):7172–7177. doi: 10.1073/pnas.96.13.7172

Figure 3.

Figure 3

The SH2 domain of SH2-Bβ is required for SH2-Bβ-induced tyrosyl phosphorylation of JAK2 and multiple other cellular proteins. (A) COS cells were cotransfected with plasmid (4 μg) encoding JAK2 and either control plasmid (10 μg) or plasmid (10 μg) encoding SH2-Bβ or SH2-Bβ(R555E). After 48 hr, cells were lysed, and proteins (40 μg) in the lysates were resolved by SDS/PAGE, immunoblotted with αPY (Top), and reprobed with αJAK2 (Middle). In a parallel experiment, proteins (40 μg) in the lysates were immunoblotted with αSH2-B (Bottom). (B) 293T cells were cotransfected with plasmid (4 μg) encoding JAK2 and either control plasmid (10 μg) or plasmid (10 μg) encoding SH2-Bβ or SH2-Bβ(R555E). After 48 hr, cells were lysed, and proteins (40 μg) in the lysates were resolved by SDS/PAGE and immunoblotted with αPY. In parallel experiments, proteins (40 μg) in cell lysates were immunoblotted with αJAK2 (Middle) or αSH2-B (Bottom). The migration of molecular weight standards (×10−3) (Top) is indicated.