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. 1999 Jun 22;96(13):7172–7177. doi: 10.1073/pnas.96.13.7172

Figure 5.

Figure 5

SH2-Bβ stimulates tyrosyl phosphorylation of Stat5B and Stat3 via JAK2. (A) COS cells were cotransfected with plasmids encoding Stat5B (5 μg), wild-type (WT) (0.5 μg) or kinase-inactive (K-E) (0.5 μg) JAK2 and SH2-Bβ (WT) (6 μg), or SH2-Bβ(R555E) (RE) (6 μg), as indicated. Twenty-four hours after transfection, cells were deprived of serum overnight. Stat5B was immunoprecipitated with αStat5B, immunoblotted with αPY (Upper), and reprobed with αStat5B (Lower). (B) 293T cells were cotransfected with plasmids encoding Stat3 (5 μg), wild-type (0.5 μg) or kinase-inactive (0.5 μg) JAK2 and SH2-Bβ (6 μg), or SH2-Bβ(R555E) (6 μg) as indicated. Twenty-four hours after transfection, cells were deprived of serum overnight. Stat3 was immunoprecipitated with αStat3, immunoblotted with αPY (Upper), and reprobed with αStat3 (Lower).