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. 1999 Jun 22;96(13):7172–7177. doi: 10.1073/pnas.96.13.7172

Figure 6.

Figure 6

SH2-Bβ enhances GH-induced tyrosyl phosphorylation of Stat5B. (A) COS cells were cotransfected with plasmids encoding GHR (5 μg), Stat5B (5 μg) and SH2-Bβ (WT) (6 μg), or SH2-Bβ(R555E) (RE) (6 μg), as indicated. Twenty-four hours after transfection, cells were deprived of serum overnight and treated with 50 ng/ml GH for 15 min. Stat5B was immunoprecipitated with αStat5B, immunoblotted with αPY (Upper), and reprobed with αStat5B (Lower). (B) COS cells were cotransfected with plasmids encoding GHR (5 μg), Stat5B (5 μg), and SH2-Bβ (6 μg), as indicated. Twenty-four hours after transfection, cells were deprived of serum overnight and treated with 50 ng/ml GH for 15 min. Proteins (40 μg) in the lysates were resolved by SDS/PAGE and immunoblotted with antiphosphoStat5 (αpStat5).