Figure 1.

Effect of the TDnaK⋅J-GrpE set and TClpB on aggregation of LDH. LDH, biotinylated for detection, was incubated in the presence of indicated components at 73°C for 30 min. The solutions were centrifuged, and supernatant (sup) and precipitate (ppt) were analyzed by polyacrylamide gel electrophoresis in the presence of sodium dodecylsulfate. Electrophoresed proteins were blotted to the membrane, and biotinylated LDH was detected by alkaline phosphatase-conjugated streptavidin. KJ, E, and B represent TDnaK⋅J complex, TGrpE and TClpB, respectively.