Figure 4.

Reactivation of heat-inactivated α-glucosidase and G6PDH mediated by the TDnaK⋅J-GrpE set and TClpB. α-glucosidase (0.2 μM) and G6PDH (0.2 μM) were incubated at 73°C for 20 min (α-glucosidase) or 10 min (G6PDH) and then subsequently incubated at 55°C. The activities of these enzymes were measured at 0 min and 60 min after the temperature shift to 55°C. Activities of α-glucosidase and G6PDH are expressed as percent of those before heat inactivation.