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Expression of LacZ by using recombinant SIN vectors. Bright-field illuminations of SIN–LacZ-injected (105 infectious particles per ml) hippocampal slice cultures fixed 1 day postinfection and stained with X-Gal. (A) Whole slice. (B) CA3 pyramidal cell. (C) Granule cells. (D) Interneuron in stratum oriens of CA3 region; arrow indicates the perimeter of the slice. Abbreviations are as in Fig. 1. [Bar = 330 μm (A), 100 μm (B and D), 80 μm (C).]
