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. 1999 Jun 22;96(13):7196–7201. doi: 10.1073/pnas.96.13.7196

Figure 2.

Figure 2

Detection of covalent DPCs with a 100-kDa protein from nuclear extracts from human colon carcinoma HCT 116, HT 29, and human leukemia CEM cells. (A) A DNA oligonucleotide (33 mer) containing Et743 preferential alkylation sequences was 5′-end-labeled on the upper strand. The oligonucleotide was incubated as indicated with nuclear extracts in the absence or presence of 0.1 μM Et743 for 1 hr at 25°C. (B) Samples were electrophoresed (8% SDS/PAGE) and analyzed by PhosphorImager. Lane 1, oligonucleotide alone; lanes 2–7, oligonucleotide + nuclear extracts from the indicated cells; lanes 3, 5, and 7, reactions with 0.1 μM Et743.