Figure 2.

Effect of the UP element on the malT core promoter in vitro. (A) EMSA for RNAP binding. The EcoRI–XbaI fragments containing the malT promoter derivatives were incubated with RNAP, treated with heparin, and analyzed by EMSA. In lane 2, CRP (50 nM) was added before RNAP. PC and NC represent the productive and nonproductive complexes, respectively. (B) In vitro transcription assay. The EcoRI–XbaI fragments containing the malT promoter derivatives were the templates, and the 205-bp EcoRI fragment containing the lacL8UV5 promoter (39) was an internal control.