Figure 5.

Effect of ribonucleotides on RNAP-promoter complexes. The EcoRI–XbaI fragments containing the malT promoter derivatives were incubated with or without CRP (50 nM) in the presence of cAMP (50 μM) and then with RNAP. The mixtures were incubated with the indicated NTPs (1 mM each) for 15 min before treatment with heparin. PC, NC, and IC represent the productive, nonproductive, and initiated complexes, respectively.