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. 1999 Jun 22;96(13):7202–7207. doi: 10.1073/pnas.96.13.7202

Figure 6.

Figure 6

Effect of the location of the UP element on promoter activity in vivo. (A) β-Galactosidase activity of the malT-lacZ fusions. The β-galactosidase activity of KI70 (Δlac) (lane 1) and OK6201 (Δlac crp−) (lanes 2–6) cells harboring the malT–lacZ fusion plasmids were measured. Each bar is expressed in Miller units (13). The value is an average obtained from three independent experiments. (B) S1 assay. Total RNAs (100 μg) prepared from KI70 (Δlac) (lane 2) and OK6201 (Δlac crp−) (lanes 3–7) cells harboring the indicated plasmids were subjected to S1 nuclease assay.