Figure 1.

Generation and identification of mice transgenic for the β₁-adrenergic receptor. (A) Transgenic vector. The complete intergenic region between the β- and α-myosin heavy chain genes (α-myosin heavy chain promoter) was ligated 5′ to the coding region of the human β₁-adrenergic receptor. At the 3′ end of the receptor cDNA, the intron and poly(A)-containing sites of the simian virus 40 T antigen were added. β, α1, α2, and α3 denote exons of the β- and α-myosin heavy chain genes. The positions of the PCR primers used to detect the transgene (B) are indicated. (B) Detection of the transgene in animals of the lines β₁TG7 and β₁TG4 by PCR with the primers indicated in A. The lanes at the right side contained 1 and 10 pg of the transgene vector in the PCR as standards and a negative control (C) without genomic DNA. αMHC, α-myosin heavy chain.