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Dependency of PGE2 formation on membrane protein concentration. Various dilutions of the membrane fraction isolated from bacteria expressing PGE synthase were incubated with PGH2 (10 μM) in the presence of glutathione (2.5 mM) for 2 min. Product formation was analyzed by RP-HPLC, and PGE2 was detected and quantified by using UV absorbance at 195 nm.
