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. Author manuscript; available in PMC: 2009 Jan 1.
Published in final edited form as: Exp Eye Res. 2007 Oct 25;86(1):150–156. doi: 10.1016/j.exer.2007.10.006

Fig. 1. SgI localization is low in the retinas of AMD donors.

Fig. 1

SgI staining is significantly reduced in all layers of the AMD retina when compared to the non-AMD eyes. Specifically, reduced labeling was observed in the photoreceptors and RPE cells. 5 control and 8 AMD donors were analyzed. Human cryosections of human donors previously diagnosed with AMD (B, C, E,) and non-AMD eyes (A, D) were probed with SgI antibody in 5% BSA, PBS and 0.3% Triton-X100 overnight at 4°C. Sections were washed, incubated with fluorophore- (A to C) or peroxidase-conjugated (D, E) secondary antibody. The controls (not shown) were omitted the antibodies and did not display any labeling. Non-AMD control retinas displayed SgI in the choroid, RPE, photoreceptor cells, cells in the inner nuclear layer, and ganglion cell layer, while in the AMD retinas SgI was mostly confined to the photoreceptor cells (B). Ch, choroid; RPE, retinal pigment epithelium, PIS, photoreceptor inner segments; POS, photoreceptor outer segments; ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer. Arrows= ganglion cells; bars (A to C)= 40μm, (D, E)=200μm.