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. Author manuscript; available in PMC: 2009 Jan 1.
Published in final edited form as: Exp Eye Res. 2007 Oct 25;86(1):150–156. doi: 10.1016/j.exer.2007.10.006

Fig. 2. SgI localization is low in the drusen of AMD donors.

Fig. 2

SgI staining is significantly reduced in the drusen from the AMD donors. 5μm paraffin sections of isolated Bruch’s membrane and choroid from a human donor previously diagnosed with AMD (E–H) and non-AMD eyes (A–D) were probed with SgI antibody in 5% BSA, PBS and 0.3% Triton-X100 overnight at 4°C. Sections were washed, incubated with secondary antibody, conjugated to biotin for 1h at RT, washed, and incubated with avidin in PBS for 30 min, then developed with DAB for 2 minutes. The controls (A, C, E, G) were omitted the antibodies and did not display any labeling. The sections were examined in bright-field (A, B, E, F) or FITC channel (C, D, G, H). Observation of the samples in the FITC channel revealed the autofluorescence of the Bruch’s membrane and drusen. 5 control and 8 AMD donors were analyzed. Bar = 200μm.