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. 1999 Jun 22;96(13):7226–7231. doi: 10.1073/pnas.96.13.7226

Figure 2.

Figure 2

Glycerol gradient purification of the U4/U6⋅U5 snRNP. (A) RNA extracted from fractions 22–27 from a 10–30% glycerol gradient was run on a 5% polyacrylamide gel and was stained with silver. Positions of the snRNAs are indicated. (B) Proteins associated with the U4/U6⋅U5 snRNP. Proteins from 64 glycerol gradient fractions were precipitated with acetone, were run on a 12% high TEMED SDS/PAGE gel, and were stained with Coomassie blue. Positions of the 24 stably associated U4/U6⋅U5 snRNP proteins are indicated.