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Mass spectrometry microsequencing spectrum from a Brr2 peptide. (A) Representative spectrum from an ≈1,158-Da tryptic peptide derived from Brr2. (B) MS/MS mass spectrometry analysis of fragmented 1,158-Da peptide. Masses calculated for the resulting ions were correlated to the entire nonredundant protein database. The labeled mass peaks for the Y′′ ions represent the protein sequence PDISSD from the Brr2 protein corresponding to amino acids 958–963.
