Table 1.
Mass spectrometric identification of U4/U6⋅U5 snRNP proteins
| Protein name | Predicted molecular mass | SGD ORF name | No. of peptides sequenced | Putative homology/functions |
|---|---|---|---|---|
| Prp8 | 280 kDa | YHR165C | 8 | Unknown |
| Brr2 | 246 kDa | YER172C | 25 | RNA helicase |
| Snu114 | 114 kDa | YKL173W | 19 | GTPase |
| Prp6 | 104 kDa | YBR055C | 18 | Zinc finger/TPR |
| Snu66 | 66 kDa | YOR308C | 13 | Unknown |
| Prp31 | 56 kDa | YGR091W | 8 | Spliceosome assembly |
| Prp3 | 56 kDa | YDR473C | 11 | Spliceosome assembly |
| Prp4 | 52 kDa | YPR178W | 8 | WD repeats |
| Prp38 | 28 kDa | YGR075C | 4 | snRNP stability |
| Snu23 | 23 kDa | YDL098C | 10 | Zinc finger |
| SmB | 22 kDa | YER029C | 4 | Sm motifs |
| Uss1/Lsm4 | 21 kDa | YER112W | 4 | Sm motifs |
| Dib1 | 17 kDa | YPR082C | 6 | Cell cycle |
| SmD1 | 16 kDa | YGR074W | 3 | Sm motifs |
| Lsm8 | 15 kDa | YJR022W | 1 | Sm motifs |
| SmD3 | 15 kDa | YLR147C | 4 | Sm motifs |
| Lsm6 | 14 kDa | YDR378C | 1 | Sm motifs |
| Snu13 | 13 kDa | YEL026W | 4 | Nhp2 homology |
| SmD2 | 12 kDa | YLR275W | 6 | Sm motifs |
| Lsm2 | 11 kDa | YBL026W | 3 | Sm motifs |
| Lsm5 | 10.4 kDa | YER146W | 2 | Sm motifs |
| SmE | 10.4 kDa | YOR159C | 2 | Sm motifs |
| SmF | 9.6 kDa | YPR182W | 2 | Sm motifs |
| SmG | 8.5 kDa | YFL017W-A | 2 | Sm motifs |
SGD, Saccharomyces Genome Database (Stanford, CA); TPR, tetratrico-peptide repeat.