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. 2007 Nov 14;2:21. doi: 10.1186/1750-9378-2-21

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Copyright © 2007 Reddout et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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HPV mRNA was expressed in vitro only after transfection. (A) RT-PCR using total RNA collected from CAL27, SCC-15 and SCC-25 non-transfectant cells does not express HPV-mRNA (representative sample of CAL27, screened for HPV16: lane 8). RT-PCR confirmed the expression of HPV18-mRNA (lane 3) and HPV16-mRNA (lane 7) from all samples (CAL27 data shown). (B) Scanning densitometry measurement of relative endpoint RT-PCR band intensities from endogenous HPV (GH354: A, lane 5) were compared to HPV16, HPV18 and HPV16/18 co-transfectants, which revealed that HPV mRNA expression in all transfectants was roughly equivalent in all samples analyzed.