Figure 3.—

Photoactivation of the con-10∷hph fusion and the endogenous con-10 gene in CHZ10 and two representative hygromycin-resistant strains. RNA was isolated from mycelia exposed to white light (10 W/m² blue light) for various periods, or kept in the dark (D). RNAs were separated by electrophoresis and hybridized with probes for con-10 (hybridizing to the mRNAs of the con-10∷hph fusion and the endogenous con-10) or tub-2 (tubulin) genes. The plots show the average and standard error of the mean of the relative photoactivation, in two independent experiments (a single experiment was performed measuring con-10 mRNA in SN1). Each hybridization signal was normalized to the corresponding tub-2 hybridization signal to correct for loading errors. Then, the hybridization signal was normalized to the signal obtained for CHZ10 after 30 min of light. Note the different scales in the relative transcription plots for SN1 and SN4. See also supplemental Figure 1 at http://www.genetics.org/supplemental/ for the light-dependent accumulation of the con-10 and con-10∷hph mRNAs in the five hygromycin-resistant strains.