Figure 5.—

Threshold of gene photoactivation in CHZ10 and the light adaptation mutants. RNA was extracted from mycelia exposed to white light of various intensities during 30 min, or kept in the dark (D). The light-exposures used were: 3.4 × 10⁴, 3.4 × 10³, and 3.4 × 10² J/m². RNAs were separated by electrophoresis and hybridized with probes for con-10 (hybridizing to the mRNAs of the con-10∷hph fusion and the endogenous con-10), con-6, or tub-2 (tubulin) genes. The plots show the average and standard error of the mean of the relative photoactivation values in two independent experiments (a single experiment was performed to detect con-6 mRNA). Each hybridization signal was normalized to the corresponding tub-2 hybridization signal, to correct for loading errors. Then, the hybridization signal was normalized to the signal obtained with CHZ10 after exposure to 30 min of light of the highest intensity.