TABLE 2.
Effects of deleting Ty1 restriction genes on Ty1 mobility and CAN1 mutagenesis
| Gene deleteda | Ty1his3-AI mobility × 10−5 (SD) | Ty1 mobility, fold increase | SUF16 insertionsa | Ty1 RNA, fold change | Ty1his3-AI RNA, fold change | Ty1 cDNA, fold change | CanR levelb |
|---|---|---|---|---|---|---|---|
| Wild type | 0.16 (0.03)c | 1 | d | 1 | 1 | 1 | 0 |
| Chromatin/transcription | |||||||
| ARG82 | 23 (11) | 144 | e | 4.4 | 16 | 13.9 | 0 |
| ASF1 | 5 (0.8) | 31 | e | 5.2 | 1.6 | 5 | + |
| BRE1 | 5.4 (0.6) | 34 | f | 1.8 | 1.4 | 4.3 | + |
| CDC73 | 2.6 (0.3) | 16 | f | 1.2 | 1.4 | 2 | 0 |
| CSE2 | 5 (0.2) | 31 | e | 1.7 | 1.3 | 4.2 | 0 |
| EAF3 | 1 (0.1) | 6 | d | 1 | 1.5 | 1 | 0 |
| ELF1 | 4 (0.4) | 25 | e | 1 | 1 | 1 | + |
| HHT1 | 1.2 (0.1) | 8 | f | 3 | 2.6 | 1 | 0 |
| LEO1 | 4.2 (0.4) | 26 | f | 1 | 1 | 3 | + |
| PAF1 | 16.2 (7.0) | 101 | e | 2.8 | 2.8 | 4.3 | + |
| RPD3 | 21 (1.4) | 131 | <WTg | 0.6 | 1.3 | 1 | 0 |
| RTF1 | 2.8 (0.6) | 17.5 | e | 1.5 | 1.3 | 2.5 | + |
| SIN3 | 0.8 (0.1) | 5 | <WTg | 0.5 | 1 | 1.4 | 0 |
| SOH1 | 5.6 (0.9) | 35 | d | 2 | 2.6 | 2.7 | 0 |
| SPT21 | 2.7 (0.5) | 17 | f | 3.4 | 3 | 3 | 0 |
| SPT5#h | 44 (10) | 275 | e | 2.8 | 8.4 | 2.5 | + |
| SRB5 | 7 (0.4) | 44 | e | 2.8 | 4.2 | 5 | + |
| Stress response | |||||||
| ASC1 | 4.4 (3.1) | 27.5 | d | 1.5 | 1.5 | 4.8 | 0 |
| IPK1 | 3.4 (0.3) | 21.3 | e | 5 | 2.8 | 4 | 0 |
| KCS1 | 2.4 (0.43) | 15 | f | 3.7 | 2.4 | 3.8 | 0 |
| MMS22 | 1.5 (0.3) | 9.4 | f | 2 | 1 | 4.2 | 0 |
| PBS2 | 0.5 (0.11) | 3 | d | 1 | 1.3 | 2 | + |
| SSK2 | 0.7 (0.02) | 4.4 | d | 1 | 1.3 | 2 | 0 |
| SSK22 | 0.6 (0.08) | 3.8 | d | 1 | 2 | 1 | 0 |
| Miscellaneous | |||||||
| AGP3 | 1.3 (1.0) | 8 | f | 1 | 1.7 | 1 | 0 |
| ALR2 | 0.7 (0.13) | 4.4 | d | 0.5 | 1 | 0.6 | 0 |
| BEM4 | 2.2 (0.6) | 13.8 | f | 2 | 2.6 | 1 | 0 |
| BUD27 | 38 (1.4) | 237.5 | f | 0.8 | 6.3 | 0.5 | 0 |
| CKB2 | 9.7 | 60.6 | f | 1.6 | 1.8 | 0.7 | 0 |
| CLN2 | 1 (0.2) | 6.3 | d | 1.4 | 2.6 | 1.7 | 0 |
| SIC1 | 1.6 (0.01) | 10 | f | 2 | 2.4 | 2.4 | 0 |
| Uncharacterized | |||||||
| YPL183C (RTT10) | 5 (0.2) | 31.3 | f | 1.7 | 2.4 | 1.9 | 0 |
a
Mutants analyzed in parallel for insertions at SUF16 are underlined (supplemental Figure S2).
b
Mutations that confer a mutator phenotype at the CAN1 locus. 0, wild-type level of CanR; +, increased level of CanR. Also refer to Figures 4 and 5 and to supplemental Tables S4–S6.
c
Average of 13 trials.
d
Wild-type level of insertions as judged by intensity of the banding pattern upstream of SUF16.
e,f
Relative increase in the intensity of the banding pattern.
g
Relative decrease in the intensity of the banding pattern. WT, wild type (also refer to supplemental Figure S2).
h
YML009W-B overlaps SPT5, designated as SPT5#.