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Construction and identification of expression vectors pET30a-1Dx5*t and pET30a-1Dx5.1*t. (A) Amplified products using the primer Pbd-1+Pbd-2 from the hybrid pGEM-T vector containing the identified genes. (B) Digestion of the hybrid pET30a-1Dx5*t with NdeI and EcoRI restriction enzymes in tracks D1-1 and D1-2. (C) Digestion of the hybrid pET30a-1Dx5.1*t with NdeI and EcoRI restriction enzymes in track D3. M, 1-kb ladder markers. Inserted fragments are indicated by arrows.
