TABLE 1.

Characteristics of the deduced mature proteins of the 1Dx5^*t and 1Dx51^*t subunits compared to previously reported HMW-GSs from Ae. tauschii and common wheat

	No. of amino acid residues and repeat unit variations
RD
Tripeptide variants			Hexapeptide variants						Nanopeptide variants
1	2	3	1	2	3	4	5	6	1	2	3	4	5	6	7	8	9	No. of Cys residues				MW (Da)
Subunits	ND	G	Q	Q	V/Ta	P	G	Q	G	Q	Q	V/Ta	G	Y	Y	P	T	S	L	Q	Q		V/Ta	V/T%b	CD	M	ND	RD	CD	Total
Dx5*t	89	1	1	1	2/20	20	10	0	13	0	16	46/70	2	0	1	2	0	0	12	2	3	14/19	67.41	42	0.91	3	0	1	4	85,782
Dx5.1*t	89	1	2	1	3/21	23	13	1	14	2	16	52/72	1	0	1	2	0	0	15	3	3	16/19	75.82	42	1.03	3	0	1	4	87,663
Dx5	89	1	1	1	2/22	20	13	1	14	3	18	50/71	2	0	1	2	0	1	15	3	4	15/19	72.22	42	1.08	3	1	1	5	87,189
Dx2	88	1	2	1	3/20	21	11	0	14	2	15	49/72	1	0	1	2	0	0	15	3	3	15/19	70.32	42	0.97	3	0	1	4	87,023
Dx2.1t	89	2	1	1	3/21	20	11	0	13	2	16	47/71	2	0	1	2	0	0	15	2	3	15/19	68.89	42	0.97	3	0	1	4	88,914
Dx2.2t	89	1	2	1	3/23	20	15	0	15	1	20	54/81	1	0	1	2	0	0	18	4	3	18/21	70.59	42	0.98	3	0	1	4	103,048
Dx2t	89	1	1	1	2/21	20	9	0	12	2	16	45/71	1	0	1	2	0	0	15	2	3	15/19	66.67	42	0.92	3	0	1	4	88,700
Dx3t	89	1	1	1	2/21	20	9	1	13	2	16	45/71	1	0	1	2	1	0	15	2	3	15/19	66.67	42	0.96	3	0	1	4	88,829
Dx5.2t	89	1	1	1	2/21	21	11	0	13	3	11	45/71	1	1	1	2	0	0	14	2	4	15/19	66.67	42	0.93	3	1	1	5	86,779
Dy12	104	—	—	—	—	18	6	3	13	6	5	30/50	1	10	2	1	8	0	10	0	2	15/19	65.22	42	1.21	5	1	1	7	68,711
Dy10	104	—	—	—	—	15	6	3	12	4	5	25/48	1	10	2	1	7	0	10	0	2	15/19	59.70	42	1.15	5	1	1	7	67,495

M, average number of variants per unit, including hexapeptides and nonapeptides. ND, N-terminal domain. RD, repetitive domain. CD, C-terminal domain. MW, molecular weights calculated from the derived amino acid sequences.

^aVariant motif number/total motif number.

^bPercentage of variant motifs relative to total motifs.