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Wild-type PIE-1 and PIE-1(DAQMEQT) inhibit transcription of a pes-10:gfp transgene. In situ hybridization shows nuclear accumulation of zygotic pes-10:gfp RNA in pie-1(zu127) embryos expressing the indicated transgenes. Arrows point to germline blastomeres, which do not accumulate pes-10:gfp RNA. Fourteen of 14 embryos expressing wild-type PIE-1 and 28/28 embryos expressing PIE-1(DAQMEQT) showed this pattern.
