Figure 5.—

GFP:PIE-1 localization in nuclei. Collapsed confocal Z-stacks through the P₂ germline blastomere show accumulation of GFP:PIE-1 in nuclei. Wild-type PIE-1 accumulates in numerous fine nuclear foci. This localization is disrupted in PIE-1 mutants that do not suppress P-Ser5 efficiently (marked with asterisk). Reductions in CDK-7, CIT-1.1/1.2, and AMA-1 (RNA polymerase II) levels also affect this localization. Note that PIE-1 also accumulates in larger cytoplasmic granules (P granules), which are visible around the nuclei in the micrographs.