TABLE 1.

Transgene	% lethality	n	% P-Ser5 (high)	n	% P-Ser2	n
No transgene	100	>50	100	>50	100	>50
PIE-1(1–335)	9	701	0	15	13	105
PIE-1(1–320)	3	1002	0	25	7	92
PIE-1(1–299)	11	298	0	25	5	50
PIE-1(1–279)	100	623	100	25	97	67
PIE-1(1–259)	100	346	ND	ND	100	25
PIE-1(1–240)	100	1038	100	25	100	151
PIE-1(DAQMEQT)	8	829	0	30	82	165
PIE-1(ΔYAPMAPT)	8	934	0	39	89	54
PIE-1(Δ240–259)	62	634	52	25	94	74

Percentage of pie-1(zu127) embryos expressing the indicated transgene that did not survive embryogenesis (% lethality), that exhibited a germline blastomere with high P-Ser5 levels (equivalent to somatic blastomeres) (% P-Ser5), or that exhibited a germline blastomere positive for P-Ser2 (% P-Ser2) is shown. Italics indicate no rescue of the pie-1(zu127) phenotypes, underlining indicates partial rescue, and no italics or underlining indicates rescue comparable to that obtained with the wild-type transgene PIE-1(1–335). Note that rescued embryos no longer have high P-Ser5 levels (equivalent to somatic blastomeres), but maintain the low intermediate levels observed in wild-type germline blastomeres (Figure 2). Examination of digital images revealed that, for the majority of pie-1(zu127) embryos expressing PIE-1(DAQMEQT) (16/20) and PIE-1(ΔYAPMAPT) (18/21), P-Ser2 levels in germline blastomeres were equivalent to P-Ser2 levels in somatic blastomeres (also see Figure 2).