Figure 1.—

meg-1 is expressed in the proximal germline and is required for fertility. (A) Diagram of early embryonic divisions highlighting P lineage. (B) In situ hybridization of meg-1 with antisense probe in wild-type gonad. No staining was observed with sense probe. Arrow marks onset of expression at mid-pachytene. Bar, 50 μm. (C) Schematic of meg-1 genomic locus showing vr10 and vr11 deletions. (D) Temperature-sensitive, maternal-effect sterility of meg-1(vr10) and meg-1(vr11) alleles. Homozygous mutants were shifted to the restrictive temperature as L4's and the progeny were assessed for sterility. Results of three experiments were averaged. Error bars indicate standard deviation.