Figure 2.—

meg-1 mutants have a severely underproliferated germline phenotype that is enhanced by meg-2(RNAi). (A–D) DIC. (A and B) meg-1(vr10) sterile adult. (C) meg-1(vr10) fertile adult, which appears very similar to wild type. (D) meg-1(vr10)meg-2(RNAi) sterile adult. (E–H) DAPI. (E) Wild-type (N2) fertile adult. (F) meg-1(vr10) sterile adult with very small distal germline. (G) meg-1(vr10) sterile adult. (H) meg-1(vr11) sterile adult. (A–H) All animals were raised at 25°; asterisks indicate the distal end of the germline, which is left of the image border in C. Approximate location of gonads is outlined. Bar, 50 μm. (I) RNAi of meg-2 was performed in a meg-1(vr10) or a meg-1(vr11) background at 20° and progeny were scored for the presence or absence of eggs to determine the percentage of sterility. Error bars, standard deviation.