Figure 3.—

meg-1 germ cells fail to proliferate extensively. (A) Number of GFP-positive germ cells in GFP∷PGL-1 and GFP∷PGL-1; meg-1(vr10) animals at 25° was scored throughout development. Larvae that would ultimately become sterile as adults could not be distinguished from those that would remain fertile, so all animals were included in the analysis. (B–E) Representative GFP images from GFP∷PGL-1 (left) and GFP∷PGL-1; meg-1 (right) at L1 (B), L2 (C), L3 (D), and L4 (E) stages. Brackets mark germ cells in the developing gonad or in one gonad arm (B and D). Boxes mark regions that are enlarged below to show germ-cell and P-granule morphology (C and E). Bar, 10 μm.