Figure 5.—

mes-1 interacts synergistically with meg-1 and meg-2. (A) Percentage of sterile progeny produced by mes-1(bn74), meg-1(RNAi), mes-1(bn74)meg-1(RNAi), meg-2(RNAi), and mes-1(bn74)meg-2(RNAi) at 15°. Error bars, standard deviation. Number of animals scored is >180 over two or more independent trials per genotype. (B–E) Embryos at two different stages stained for DAPI (blue), anti-PGL-1 (green), and anti-MES-1 (red). (B and C) Wild type. (D and E) meg-1(vr11). Arrow indicates region of normal MES-1 localization. Arrowheads indicate ectopic MES-1 localization. Bar, 10 μm. (F and G) mes-1(bn74) 4-cell (F) and ∼24-cell (G) embryos at 25° stained with DAPI (blue), anti-PGL-1 (green), and anti-MEG-1 (red).