TABLE 3.
Percentage of sterile progeny and germline development in P-granule mutants
| Germline development
|
||||
|---|---|---|---|---|
| Genotype | % sterile progeny (n) | % severely affected | % moderately affected | n |
| glh-1 | 3 (62) | 0 | 100 | 17 |
| pgl-1 | 85 (55) | 15 | 85 | 13 |
| meg-1 | 53 (193) | 70 | 30 | 10 |
| glh-1;meg-1 | 100 (38) | 91 | 9 | 11 |
| glh-1;pgl-1 | 100 (21) | 45 | 55 | 11 |
| pgl-1;meg-1 | 77 (156) | 26 | 74 | 19 |
| glh-1;pgl-1;meg-1 | 96 (28) | 90 | 10 | 10 |
L4 hermaphrodites were shifted from 20° to 25°, and progeny were raised at 25° and scored for sterility as described in materials and methods. Sterile adults were fixed and stained with DAPI to assess germline development. Underlining highlights meg-1 and pgl-1 comparison.