TABLE 1.

The somatic gonad is required for DAF-16 to activate some of its target genes in animals that lack germ cells

Construct	Construct no.	Strain	Trial	Intact	n	Germ cell (−)	n	P	Whole gonad (−)	n	P	P′
sod-3∷gfpa	muIs84	CF1553	1	1 ± 0.048	34	8.0 ± 1.17	26	<0.001	1.6 ± 0.12	32	<0.001	<0.001
gpd-2∷gfp	sEx11128	BC11128	1	1 ± 0.12	19	2.0 ± 0.185	17	<0.001	0.53 ± 0.073	16	0.002	<0.001
			2	1 ± 0.076	28	0.91 ± 0.12	26	<0.001	0.60 ± 0.042	32	<0.001	0.031
			3	1 ± 0.12	19	1.47 ± 0.18	13	0.04	0.76 ± 0.14	18	0.21	0.004
dod-8∷gfp	sIs10314	CF2630	1	1 ± 0.007	28	2.17 ± 0.026	38	<0.001	2.3 ± 0.017	32	<0.001	0.60
			2	1 ± 0.11	36	15.9 ± 1.5	35	<0.001	9.9 ± 0.49	46	<0.001	<0.001
			3	1 ± 0.10	30	3.3 ± 0.35	27	<0.001	2.5 ± 0.15	33	<0.001	0.033
			4	1 ± 0.086	31	3.1 ± 0.22	34	<0.001	2.2 ± 0.091	35	<0.001	<0.001
dod-8∷rfp	muEx405	CF2760	1	1 ± 0.13	27	2.8 ± 0.19	26	<0.001	1.9 ± 0.14	38	<0.001	<0.001
			2	1 ± 0.12	34	4.2 ± 0.40	37	<0.001	4.2 ± 0.35	43	<0.001	0.92
			3	1 ± 0.17	28	4.8 ± 0.34	35	<0.001	4.0 ± 0.44	21	<0.001	0.14
			4b	1 ± 0.16	20	4.8 ± 0.40	18	<0.001	2.9 ± 0.40	17	<0.001	0.002
			5c	1 ± 0.13	13	2.2 ± 0.19	16	<0.001	2.2 ± 0.23	12	<0.001	0.93
nnt-1∷gfp	sEx10466	BC10466	1	1 ± 0.25	23	6.0 ± 0.92	23	<0.001	5.1 ± 0.92	23	0.002	0.57
			2	1 ± 0.23	34	3.5 ± 0.43	29	<0.001	4.2 ± 0.47	35	<0.001	0.25
			3	1 ± 0.14	30	9.1 ± 1.0	32	<0.001	6.4 ± 0.80	29	<0.001	0.04
			4d	1 ± 0.27	31	9.6 ± 1.4	23	<0.001	11 ± 1.7	25	<0.001	0.51

Values represent mean fluorescence intensity relative to intact controls. Fluorescence was measured on day 2 of adulthood. P represents the P-value (Student's t-test) compared to intact control, and P′ represents the P-value compared to animals lacking germ cells (Z2 and Z3 ablated).

^aChanges in Psod-3∷gfp expression were observed twice by eye and quantification was performed on the third trial.

^bSame data labeled trial 1 of dod-8∷rfp in Table 2.

^cSame data labeled trial 2 of dod-8∷rfp in Table 2.

^dSame data labeled trial 2 of nnt-1∷gfp in Table 2.