TABLE 2.

daf-16 dependence of gene expression in animals lacking germ cells

Construct	Construct no.	Strain	Trial	daf-16 genotype	Intact	n	P	Germ cell (−)	n	P	Whole gonad (−)	n	P	P′
sod-3∷gfp	muIs84	CF1874	1	daf-16(mu86)	1 ± 0.044	29	—	0.79 ± 0.044	26	0.001	0.87 ± 0.042	24	0.034	0.17
gpd-2∷gfp	sEx11128	BC11128	1	daf-16(RNAi)	1 ± 0.087	20	—	0.84 ± 0.13	19	0.301	0.66 ± 0.11	18	0.019	0.29
		BC11128		daf-16(+)	0.9 ± 0.077	19	0.42	1.5 ± 0.16	17	0.005
		BC11128	2	daf-16(RNAi)	1 ± 0.087	25	—	0.55 ± 0.11	16	<0.001	0.24 ± 0.062	27	<0.001	0.14
		BC11128		daf-16(+)	0.63 ± 0.068	25	0.002	1.8 ± 0.12	21	<0.001
dod-8∷gfp	sIs10314	CF2676	1	daf-16(mu86)	1 ± 0.077	24	—	1.8 ± 0.14	17	<0.001	2.3 ± 0.15	27	<0.001	0.016
		CF2630		daf-16(+)	1.3 ± 0.077	30	0.006
		CF2676	2	daf-16(mu86)	1 ± 0.19	21	—	2.7 ± 0.44	18	0.002	4.1 ± 0.39	9	<0.001	0.023
		CF2630		daf-16(+)	1.1 ± 0.15	24	0.67	8.6 ± 0.69	25	<0.001
dod-8∷rfp	muEx405	CF2922	1	daf-16(mu86)	1 ± 0.14	17	—	2.3 ± 0.29	24	<0.001	1.6 ± 0.19	28	0.011	0.07
		CF2760		daf-16(+)a	0.84 ± 0.13	20	0.42	4.0 ± 0.33	18	<0.001	2.4 ± 0.33	17	<0.001
		CF2922	2	daf-16(mu86)	1 ± 0.12	12	—	2.0 ± 0.30	13	0.009	2.6 ± 0.42	17	0.001	0.19
		CF2760		daf-16(+)b	1.7 ± 0.23	13	0.016	3.6 ± 0.32	16	<0.001	3.7 ± 0.38	12	<0.001
nnt-1∷gfp	sEx10466	BC10466	1	daf-16(RNAi)	1 ± 0.21	19	—	1.5 ± 0.23	28	0.125	2.2 ± 0.26	40	<0.001	0.03
		BC10466		daf-16(+)	1.2 ± 0.24	16	0.48	2.8 ± 0.41	17	<0.001
		CF2923	2	daf-16(mu86)	1 ± 0.17	35	—	2.6 ± 0.32	39	<0.001	2.7 ± 0.40	33	<0.001	0.88
		BC10466		daf-16(+)c	0.56 ± 0.15	31	0.05	5.4 ± 0.787	23	<0.001	6.2 ± 0.96	25	<0.001

Values represent mean fluorescence intensity relative to intact daf-16(−) controls. Fluorescence was measured on day 2 of adulthood. P represents the P-value (Student's t-test) compared to intact control, and P′ represents the P-value compared to animals lacking germ cells (Z2 and Z3 ablated).

^aSame data labeled trial 4 for dod-8∷rfp in Table 1.

^bSame data labeled trial 5 for dod-8∷rfp in Table 1.

^cSame data labeled trial 4 for nnt-1∷gfp in Table 1.