TABLE 4.

Relative levels of Psod-3:gfp expression in daf-2 mutants subjected to germline or whole-gonad ablation

Strain	Genotype		Psod-3∷gfp expression relative to intact	n	P	P′	Mean life span (days)	n	P	P′
CF1580	daf-2(e1370)	Intact	1 ± 0.042	23	—		43.2 ± 0.93a	245	—
		Germ cell (−)	1.52 ± 0.063	17	<0.001		64.5 ± 3.5a	59	<0.001
		Whole gonad (−)	1.98 ± 0.083	17	<0.001	<0.001	69.5 ± 6.0a	31	<0.001	0.33
CF2533	daf-2(e1368,RNAi)	Intact	1 ± 0.19	14	—		51.0 ± 1.9b	68	—
		Germ cell (−)	1.17 ± 0.13	11	0.456		87.90 ± 4.4	37	<0.001
		Whole gonad (−)	1.76 ± 0.18	15	0.007	0.014	124.1 ± 5.9b	39	<0.001	<0.001
CF1553	daf-2(RNAi)	Intact	1 ± 0.19	18	—		36.5 ± 1.7	113	—
		Germ cell (−)	5.15 ± 0.60	16	<0.001		72.8 ± 2.5	73	<0.001
		Whole gonad (−)	3.09 ± 0.60	21	0.003	0.020	58.2 ± 2.2	80	<0.001	<0.001
CF2533	daf-2(e1368)	Intact	1 ± 0.034	37	—		34.3 ± 1.0a	110
		Germ cell (−)	3.74 ± 0.19	35	<0.001		71.0 ± 2.3a	45	<0.001
		Whole gonad (−)	3.48 ± 0.19	40	<0.001	0.37	41.1 ± 1.5a	45	<0.001	<0.001
CF1588	daf-16(mu86); daf-2(e1370)	Intact	1 ± 0.044	25	—
		Germ cell (−)	0.74 ± 0.029	28	<0.001
		Whole gonad (−)	0.93 ± 0.042	25	0.26	<0.001
CF2683	daf-16(mu86); daf-2(e1368)	Intact	1 ± 0.067	19	—
		Germ cell (−)	0.85 ± 0.064	15	0.11
		Whole gonad (−)	0.88 ± 0.044	11	0.14	0.69

Psod-3∷gfp expression values represent mean fluorescence intensity relative to intact controls. P represents the P-value (Student's t-test for Psod-3∷gfp expression and Logrank Mantel/Cox for life span data) compared to intact control, and P′ represents the P-value compared to animals lacking germ cells (Z2 and Z3 ablated).

^aLife span data previously published in Hsin and Kenyon (1999), and included here for comparison.

^bLife span data previously published in Arantes-Oliveira et al. (2003) and included here for comparison.