Figure 6.
(Upper) Effect of 17β-E2 and genistein on proliferation of adult rat vascular SMCs to 20 ng/ml PDGF-B as quantitated by 3H-TdR incorporation. Shown is one representative experiment of three. Triplicate determinations are ±SD. (Lower) The effect of 17β-E2 and genistein on the migration of rat vascular SMCs. Shown is one representative experiment of two. The cells were plated on the upper chambers with 17β-E2 or genistein and were stimulated with 60 ng/ml of PDGF-BB in the lower chamber. The effect of the estrogenic compounds on migration rate (number of cells in the lower surface of the filter) was quantitated after 24 h with a light microscope using 400× magnification.