TABLE 4.

Data summary

	URA3	CAN1
Sequenced mutations	237	227
Mutations at locus	207	227
Fraction at locus	0.87	1.00
Mutation rate to resistance	5.43	15.2
Rate of loss of function of locus	4.74	15.20
Nonsense mutations found	64	70
Fraction of total mutations	0.31	0.31
No. of unique nonsense mutations	42	54
Fraction of possible unique nonsense mutations observed	0.34	0.24
Missense mutations found	103	80
Fraction of total mutations	0.50	0.35
No. of unique missense mutations	66	63
Insertion/deletion/other mutations found	40	77
Fraction of total mutations	0.19	0.34
Base pairs	804	1773
Possible substitutions (bp × 3)	2412	5319
Possible nonsense substitutions	123	226
Possible nonsense substitutions leading to resistancea	123	226
Fraction of nonsense substitutions leading to resistancea	1	1
Possible missense substitutions	2289	5093
Possible missense substitutions at URA3 leading to resistance	193
Possible missense substitutions at CAN1 leading to resistance		264
Fraction of missense substitutions leading to resistance	0.08	0.05
Target size for loss of function via base pair substitution (bp)	104	163
Target size for loss of function via insertion/deletion/otherb (bp)	804	1773
Effective target size (bp)	125	236
Phenotypic mutation rate (10−8)	5.43	1.52
Mutation rate at locus (10−8)	4.75	1.52
Mutation rate per base pair at locus (10−10)	3.80	6.44

^aWe assume that all nonsense mutations result in loss of function.

^bWe assume that all insertion/deletion/other mutations result in loss of function; therefore the target size for these mutations is the size of the gene.