Figure 2.—

cin-4 is necessary for centromere resolution. Nuclei in prometaphase from one- or two- cell embryos of (A) wild type, (B) cin-4(mr127), and (C) cin-4(mr127);smc-1(e879). Cell-cycle position was inferred by staining with the mAb414 antibody (not shown) to mark nuclear envelope breakdown. Embryos were obtained from cin-4(mr127) hermaphrodites after a 6-hr shift to the nonpermissive temperature and stained for DNA, CENP-A/HCP-3 (red in merged image), and CENP-C/HCP-4 (green in merged image). Colocalization between CENP-A/HCP-3 and CENP-C/HCP-4 was observed by yellow in the merged image. (D) Quantitative analysis of the incidence of resolved sister kinetochores. The number of chromosomes examined is in parentheses. Bar, 5 μm.