TABLE 1.
Efficiency of RNAi experiments determined by RT–qPCR
| Fold decrease in mRNA levels ±SE (P-value)
|
|||
|---|---|---|---|
| Primary target locus | cin-4 | top-2 | R05D3.1 |
| cin-4(RNAi) | 9.5 ± 1.2 (0.01) | 4.0 ± 1.1 (0.03) | 1.2 ± 0.1 (0.14) |
| top-2(RNAi) | 1.0 ± 0.1 (0.5) | 10.9 ± 0.8 (0.01) | 1.1 ± 0.1 (0.18) |
| R05D3.1(RNAi) | 1.0 ± 0.1 (0.14) | 1.0 ± 0.3 (0.08) | 8.0 ± 1.0 (0.02) |
CT values for target cDNAs were normalized to the control cDNA, F22B2.13, to correct for minor differences in cDNA concentrations. The fold decrease in expression of target cDNAs relative to a mock(RNAi) was calculated using the ΔΔCT method. P-values were calculated using the Student's t-test. SE, standard error.